Engineering physiologically relevant microenvironments is critical foradvancing organoid research in tissue engineering and regenerative medicine.Despite recent progress, challenges remain in achieving reproducibility, scalability,and biological relevance of organoids. To address this, we introduce NestWell®, apermeable microwell platform with a 3D geometrically engineered nanofibrousmembrane that enables precise control of organoid size and ensures unconstraineddelivery of nutrients and differentiation signals. In parallel, OrgaNest®, an automatedculture system, enhances reproducibility, reduces culture costs while supportingscalable organoid production.
